The construct of cell adhesion sites and degradation space in alginate hydrogels to enhance spreading and osteogenic differentiation of MG-63 cells
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DOI:
10.7502/j.issn.1674-3962.2013.10.07
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Abstract:
Adhesion and spreading of anchorage-dependent cells are two essential factors for their subsequent settlement and commitment in 3D hydrogel substrate. An adhesive macroporous alginate hydrogel system was suggested here to overcome the low cell affinity and high cell constraint of hydrogel substrates for cells encapsulated in them. RGD peptide was grafted onto the alginate molecule to support cell adhesion and gelatin microspheres were introduced into photocrosslinked alginate hydrogels to create macropores by their degradation at 37℃ for cell spreading and facilitate cell ingrowth as well as prolonged cell survival within hydrogels. With the incorporation of gelatin microspheres, the hybrid alginate hydrogels exhibited significantly enhanced mechanical properties and decreased swelling ratios. MG-63 cells in alginate hydrogel cooperated with RGD and gelatin microspheres exhibited a rapid proliferation and spread gradually with prolonged culture time, and their osteogenic differentiation was greatly facilitated, such as the high expression of BMP-2, COL-I and OCN genes. While in the pure alginate hydrogel (ALG) and hydrogels only capable of cell affinity (RGD-ALG), cells could just exhibit either a rounded shape or a spreading morphology with a very limited degree, and the osteogenic differentiation was also inhibited