（1. Department of Emergency, Changzheng Hospital, The Second Military Medical University （2. National Engineering Research Center for Nanotechnology
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DOI:
10.7502/j.issn.1674-3962.2016.06.09
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Abstract:
Artificial pulmonary surfactant (APS)/Perfluorocarbon (PFC) emulsion using APS as a carrier is prepared through highspeed emulsification, the effect of APS/PFC emulsion on cell proliferation and its ability of improving pulmonary oxygenation to seawater drowning rabbits are explored. PFC content of APS/PFOB freezedried powder is 23.54%. A549 cell viability is increased gradually with the increasing concentration of APS/PFOB emulsion under condition of 1% oxygen concentration. About 124% cell proliferation rate is maintained when the concentration is 5 mg/mL. Moreover, it is demonstrated that APS/PFOB emulsion can improve pulmonary oxygenation to seawater drowning rabbits through pulmonary delivery. APS/PFC emulsion has abilities of promoting A549 cell proliferation during hypoxia and improving pulmonary oxygenation to seawater drowning rabbits through pulmonary delivery, it has potential application for improving lung ventilation.