This paper used potentiometric titration and NaOH standard solution to determine the concentration of hydrazine nitrate solution. The method was evaluated from linearity, precision and accuracy, and was compared with the capacity method by F-test and t-test. The results showed that potentiometric titration method could be used to determine the concentration of hydrazine nitrate. The linear relationship parameter R2 was 0.999 9 when the concentration is in the range of 0.05~1.50 mol/L; the relative standard deviation was 0.166 7% when testing the same solution for 5 times. And testing relative error less than 1.5%, when the consumption of titration liquid was more than 0.5 mL. There was no significant difference in measurement values between potentiometric titration and the capacity method. Through the analysis of the disturbing factors, it was found that the temperature had no effect on the method. However, when ammonium nitrate was present in the tested solution, it might have a great influence on the test results. Acetone can be used to separate ammonium nitrate and hydrazine nitrate.