(1. Department of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China) (2. Shenzhen Jinglai New Material Technology Co, Ltd, Shenzhen 518000, China) (3. Guangdong Welding Institute, Guangzhou 510650, China) (4. Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China)
Integrating biomimetic micro-nano structure on implants surface can provide a favorable microenvironment for organism cell, so as to effectively increase the cellular activity and improve tissue repairing. However, there are few appropriate bone tissue engineering materials which could simultaneously simulate native bone in the aspect of composition and structure at present. Therefore, biomimetic bone tissue engineering materials with large-scale controllable micro-nano structures were prepared by combining biomineralization and micro/nano-fabrication in this work. Moreover, it was the first time to prove that their composition and controllable ordered micro-nano structure could facilitate the regeneration of vascularized bone. The results have shown that, firstly, mineralized hydroxyapatite nanoparticles (HANPs) with different microstructures were synthesized by different composite polymer templates. And, large-scale ordered micropatterns were integrated on the biomimetic bone tissue engineering materials by micro-contact method. Secondly, rat mesenchymal stem cells (rMSCs) cultured on the surface of biomimetic bone tissue engineering materials present highly orientated alignment. The angiogenesis and osteogenesis expressions of rMSCs increase. Meanwhile, we also found the biomimetic bone tissue engineering materials promoting vascularized intramembranous ossification via a rat skull defect model. This work provides an advanced platform for manipulating directional differentiation of stem cells, as well as gives a theoretical and practical foundation for preparing novel biomimetic bone tissue engineering materials with micronano structures. It is a potential application for this materials in clinic.