Tissue engineering technology is a vital method to develop biological substitutes that restore damaged tissues. The porous networks of hydrogels with resemblance to human tissues make them promising candidates for tissue engineering. Injectable hydrogels with ease of handling and complete filling of irregular defects can be implanted in bodies through minimally invasive surgical procedures, which reduces administration risks effectively. Natural polysaccharide-based injectable hydrogels are especially attractive as medical implanting biomaterials due to their excellent biocompatibility and biodegradability. In recent years, injectable hydrogels based on natural polysaccharide materials have been paid much attention. The fabrication methods and applications in tissue engineering of natural polysaccharide-based injectable hydrogels have been widely studied. This review summarized typical natural polysaccharide materials and crosslinking mechanisms of natural polysaccharide-based injectable hydrogels. Meanwhile, the progress of functionality in high-strength and 3D bioprinting was reviewed. Finally, future challenges and perspectives of natural polysaccharide-based injectable hydrogels in tissue engineering were proposed．