As one of the three elements of tissue engineering, the important role of biomaterials in tissue engineering and regenerative medicine has received increasing attention. Biomaterials are not only structural supports and delivery vehicles for cells and growth factors, but also suitable regenerative microenvironments that regulate the cellular behavior of stem cells, immune cells, tissue-specific cells, and active tissue regeneration potentials. The intrinsic physical and chemical properties, biological activity, and exogenous stimuli transmitted by biomaterials can regulate cell behavior, activate gene expression, and induce tissue regeneration. Based on the principles of bionic design, decoding the material signals of natural extracellular matrix and revealing its regulatory effects and molecular mechanisms on cell behaviors, guiding the design of multi-functional biomaterials, and inducing tissue regeneration have become a common scientific issue in tissue engineering and regenerative medicine. Therefore, we propose a biomaterial-based multimodal tissue engineering strategy, which is to design and develop "All-in-One" multi-functional biomaterials to deliver multimodally cell-regulatory signals,synergistically regulating cell fate and inducing tissue regeneration.Here, we first reviewed the regulatory roles of some typical materials signals on cell behaviors, and then introduced the application of biomaterial-based multimodal tissue engineering strategy in neural tissue engineering as an example. Finally, the prospects and challenges of future researches were also proposed