1. School of Materials Engineering, Shanxi College of Technology, Shuozhou 036000，China
2. School of Electric Power, North University of China, Shuozhou 036000，China
et al.
Based on Cu-MOF conductive materials, an in-situ electrochemical fabrication method for Ru3+@Cu-MOF(-NH2)/Au interface was established and its electrochemical properties were fully characterized. Utilizing RuCl3 as the central molecule and 2-aminoterephthalic acid as the ligand, the Ru3+@CuMOF(-NH2)/Au was formed through electrochemical anodic synthesis. The electrochemical response performance of the interface to methylene blue (MB) was investigated by square wave voltammetry (SWV) and cyclic voltammetry (CV). The results show that the detection limit of the interface to MB is 4.2 pmol/L, and the linear range is 10.0 pmol/L~100.0 nmol/L, which proves that the interface has excellent electrochemical response performance to MB. After applying it to the electrochemical aptasensor for Kanamycin (Kan-A), it’s found that the sensitivity of the sensor to Kan-A is 0.06, the detection limit is 12.3 pmol/L, and the linear range is 10.0 pmol/L~100.0 nmol/L. The sensor can significantly distinguish the four antibiotics of penicillin (Pen-G), tetracycline (TET), amoxicillin (AMO) and sulfamethoxypyrimidine (SMZ), showing excellent electrochemical sensing performance and can be used for the quantitative detection of Kan-A in the actual system.