文章信息/Info

Title:

Recombinant fusion protein based biomaterials for tissue engineering

作者:

張妍 許可 高超 楊軍*

南開大學生命科學學院生物活性材料教育部重點實驗室,天津300071

Author(s):

ZHANG Yan;  XU Ke;  GAO Chao;  YANG Jun*

State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin 200071, China

關鍵詞:

融合蛋白; 組織工程; 細胞外基質; 細胞外微環境; 血管化; 干細胞

DOI:

10.7502/j.issn.1674-3962.2015.03.03

文獻標志碼:

A

摘要:

仿生生物體組織的結構與狀態，研制細胞與支架材料三維復合體，將有望開發出用于修復受損組織和器官的生物替代品。細胞的正常生物活動需要來自細胞外微環境中的信號分子持續刺激。作為人工細胞外基質的重要組成部分，材料的物理及化學信號能夠影響細胞基因及蛋白表達，但遠不足以達到體外重建組織器官的長遠目標。利用基因與蛋白質工程技術對天然蛋白結構進行融合改造，設計并生物合成具有多重結構與功能的融合蛋白生物材料,可用于改善天然或化學合成生物材料的生物活性。本文綜述了國內外基于融合蛋白的生物材料的發展動態，概括了融合蛋白的設計思路與制備技術，并重點詳述了細胞生長因子以及細胞黏附因子相關融合蛋白在組織工程領域的應用。

Abstract:

The construction of 3D biological substitutes consisting of cells and scaffolds is an expected approach for tissue and organ restoration. Normal biological activities of cells requires the continuous stimulation of signaling molecules from the extracellular microenvironment. As an important part of the artificial extracellular matrix, both chemical signals and physical forces of the biomaterials remodeling regulate cell gene and protein express but far not enough to sculpt tissue structure. Using gene and protein engineering technology, a fusion protein could be designed with multiple functions and improved the biological activities of natural or synthetic materials. In this article, we summarize the developments of biomaterials based on the fusion protein, as well as the concepts of fusion protein design and preparation. Meanwhile, we highlight the applications of fusion proteins that related to cell growth factors and cell adhesion factors in tissue engineering.