文章信息/Info

Title:

The development of micro/nano particles and hydrogel based vaccine delivery system

作者:

岳華;  馬光輝

中國科學(xué)院過程工程研究所生化工程國家重點(diǎn)，北京 100190

Author(s):

YUE Hua;  MA Guanghui

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

關(guān)鍵詞:

納微米顆粒; 凝膠; 佐劑; 疫苗傳遞系統(tǒng)

DOI:

10.7502/j.issn.1674-3962.2015.03.04

文獻(xiàn)標(biāo)志碼:

A

摘要:

新型疫苗的飛速發(fā)展對佐劑或遞送體系的研究提出了巨大的挑戰(zhàn)。針對傳統(tǒng)佐劑在細(xì)胞免疫應(yīng)答方面不足的問題，選取生物相容性好的高分子材料為主要基質(zhì)，設(shè)計并制備出一系列尺寸均一且具有不同顆粒性質(zhì)（粒徑、電荷或表面基團(tuán)）的納微米顆粒佐劑。研究表明，新型顆粒佐劑表現(xiàn)出良好的增加抗原內(nèi)化、促進(jìn)抗原提呈和T淋巴細(xì)胞增殖等優(yōu)勢，有望滿足重要傳染性疾病甚至惡性腫瘤的防治需求。此外，針對目前仍無有效黏膜免疫佐劑的難題，以具有生物粘附性的殼聚糖為基質(zhì)，開發(fā)了溫度敏感性凝膠作為疫苗佐劑。該凝膠佐劑不僅可以使鼻黏膜制劑有效鋪展和停留，還可以借助材料本身特性增加抗原滲透性并活化T細(xì)胞，從而誘導(dǎo)高效的免疫應(yīng)答，拓展了傳統(tǒng)以注射為主的免疫遞送方式。上述基于納微米顆粒和凝膠佐劑的疫苗傳遞體系的系統(tǒng)性研究，為新型疫苗傳遞系統(tǒng)的開發(fā)提供了有意義的探索。

Abstract:

The development of novel vaccines posed several challenges for the study of adjuvant. To overcome the incapability of conventional adjuvant on cellular immune response, a serious of uniform sized micro/nano-particles (MP/NPs) with different physio-chemical properties (size, charge or surface group) were prepared by using the polymer materials with good biocompatibility. MP/NPs could improve the antigen internalization, antigen presentation and T cell proliferation, which were catering for the preventive or therapeutic effect against the infectious diseases or cancer. To complement the nasal immune adjuvant, a novel thermal-sensitive hydrogel, which can undergo sol-gel transition at body temperature were developed by using the chitosan with good bioadhesion. This hydrogel could significantly prolong the antigen residence time, enhance the antigen penetration, and activate the immune cells, thus inducing strong immune responses and expanding the conventional vaccination by injection. The MP/NPs and hydrogel based adjuvant exhibited a promising era for the novel vaccine delivery system.