文章信息/Info

Title:

Microscale tissue engineering and the bottom-up assembly for 3D tissue construction

作者:

肖文謙; 孫靜; 范紅松*

四川大學國家生物醫學材料工程技術研究中心

Author(s):

 XIAO Wenqian' target="_blank" rel="external">"sans-serif"">XIAO Wenqian;  SUN Jing and FAN Hongsong

National Engineering Research Center for Biomaterials, Sichuan University

關鍵詞:

組織工程; 微尺度組織工程; 微制造; 水凝膠; 組裝

DOI:

10.7502/j.issn.1674-3962.2015.07.13

文獻標志碼:

A

摘要:

自然組織是由高度復雜化的組織微單元結構體組成，如何在體外重現這些微結構體的結構和功能是現今組織工程學的巨大挑戰。微尺度組織工程是一項利用現代微制造技術加工和控制支架材料的微觀空間特征從而精確控制細胞微環境，在微尺度水平上引導和促進制備具有模擬天然組織的非同質性和各向異性的人造組織工程復合體的新型組織工程構建技術。微尺度組織工程通過制備功能化微結構單元并通過“自下而上”途徑構建路徑使得制備出具有復雜精細結構的工程化組織成為可能，也為組織工程提供了一條新的思路。本文對微尺度組織工程的常用技術和構建方式做了詳細介紹，重點闡述了通過微凝膠單元制造和自下而上的組裝構建方式制備組織工程復合體的新技術，并對該領域的未來應用和挑戰進行了展望。

Abstract:

Since most living tissues are composed of repeating micro-units with highly complex organizational structure, one of the major challenges in tissue engineering is to reproduce the architectures and functional properties of these micro-units in vitro. Microscale tissue engineering is a new emerging field which can precisely control the cellular microenvironment by the regulation of the microscopic geometry of scaffold using modern microfabrication technology. Accordingly, the simulation of natural tissues with non-homogeneous and isotropic heterosexual artificial can be directed and promoted on the microscale level. Through fabrication the micro-units and assembly by the “bottom-up” approach, construction the engineered tissue with complicated structure became possible .Therefore the mciroscale tissue engineering could provide us a new pathway and idea for the tissue engineering. In this paper, an overview of microfabrication technologies and construction approaches common used in microscale tissue engineering were presented, and the future applications and challenges of this field were discussed. Meanwhile, a bottom-up approach to direct the assembly of cell-laden microgels to generate tissue constructs was illustrated.