文章信息/Info

Title:

One-Pot Synthesis and Bioimaging Application of Ethanolamine-Grafted Graphene Quantum Dots

作者:

曾敏翔; 陳 翔; 謝虞清; 管 劍; 甄杰明; 朱先軍; 楊上峰

中國科學技術大學材料科學與工程系 中國科學院能量轉換材料重點實驗室 合肥微尺度物質科學國家實驗室，安徽 合肥，230026

Author(s):

ZENG Minxiang; CHEN Xiang; XIE Yuqing; GUAN Jian; ZHEN Jieming; ZHU Xianjun; YANG Shangfeng

關鍵詞:

石墨烯; 量子點; 光致發光; 生物成像

分類號:

O69

DOI:

10.7502/j.issn.1674-3962.2015.11.06

文獻標志碼:

A

摘要:

作為新一代基于碳材料的量子點，乙醇胺（Ethanolamine，ETAM）修飾的石墨烯量子點(ETAM-GQDs)成功地通過一步水熱法被合成出來，并通過實驗顯示出在生物成像應用中的潛力。以檸檬酸作為碳源、甘氨酸作為橋聯劑，通過乙醇胺/去離子水共溶劑水熱法，成功地實現了在石墨烯量子點表面修飾乙醇胺得到ETAM-GQDs。通過原子力顯微鏡（AFM）、光電子能譜（XPS）、拉曼光譜等對ETAM-GQDs進行表征，在測得ETAM-GQDs的穩態熒光光譜后，通過使用硫酸奎寧作為參比，在365 nm紫外光激發下測得的ETAM-GQDs的量子產率為38.2%。除此之外，活體細胞HL7702和ETAM-GQDs共培養后，通過熒光成像實驗證實了ETAM-GQDs可以作為有效的生物成像劑。

Abstract:

Ethanolamine-grafted graphene quantum dots (ETAM-GQDs) as a novel type of carbon-based quantum dots have been successfully synthesized via a facile one-pot hydrothermal approach，and show potential application in bioimaging.The hydrothermal synthesis of ETAM-GQDs was accomplished by introducing ethanolamine (ETAM) as the co-solvent blending deionized water while citric acid and glycine are used as the main carbon source and bridge for condensation respectively. The size distribution of ETAM-GQDs was determined by AFM and TEM. The photoluminescent (PL) quantum yield of ETAM-GQDs was measured to be 38.2% under 365 nm light excitation by using quinine sulphate as a standard. The biocompatibility of ETAM-GQDs evaluated by MTT assay indicated the low toxicity of ETAM-GQDs at concentrations below 1 mg/mL. Fluorescent imaging of living HL7702 cells incubated with ETAM-GQDs demonstrated that ETAM-GQDs can be applied as an effective bioimaging agent.