文章信息/Info

Title:

One-Pot Synthesis and Bioimaging Application of Ethanolamine-Grafted Graphene Quantum Dots

作者:

曾敏翔; 陳翔; 謝虞清; 管劍; 甄杰明; 朱先軍; 楊上峰

中國科學(xué)技術(shù)大學(xué)材料科學(xué)與工程系，中國科學(xué)院能量轉(zhuǎn)換材料重點(diǎn)實(shí)驗室，合肥微尺度物質(zhì)科學(xué)國家實(shí)驗室

Author(s):

ZENG Minxiang;  CHEN Xiang;  XIE Yuqing;  GUAN Jian;  ZHEN Jieming;  ZHU Xianjun;  YANG Shangfeng

關(guān)鍵詞:

石墨烯; 量子點(diǎn); 光致發(fā)光; 生物成像

文獻(xiàn)標(biāo)志碼:

A

摘要:

作為新一代基于碳材料的量子點(diǎn)，乙醇胺（Ethanolamine，ETAM）修飾的石墨烯量子點(diǎn)(ETAM-GQDs)成功地通過一步水熱法被合成出來，并通過實(shí)驗顯示出在生物成像應(yīng)用中的潛力。以檸檬酸作為碳源、甘氨酸作為橋聯(lián)劑，通過乙醇胺/去離子水共溶劑水熱法，成功地實(shí)現(xiàn)了在石墨烯量子點(diǎn)表面修飾乙醇胺得到ETAM-GQDs。通過原子力顯微鏡（AFM），光電子能譜（XPS），拉曼光譜等對ETAM-GQDs進(jìn)行了表征。在測得ETAM-GQDs的穩(wěn)態(tài)熒光光譜后，通過使用硫酸奎寧作為參比，在365nm紫外光激發(fā)下測得的ETAM-GQDs的量子產(chǎn)率為38.2%。除此之外，活體細(xì)胞HL7702細(xì)胞和ETAM-GQDs共培養(yǎng)后，通過熒光成像實(shí)驗證實(shí)了ETAM-GQDs可以作為有效的生物成像劑。

Abstract:

Ethanolamine-grafted graphene quantum dots (ETAM-GQDs) as a novel type of carbon-based quantum dots have been successfully synthesized via a facile one-pot hydrothermal approach, and show potential application in bioimaging. The hydrothermal synthesis of ETAM-GQDs was accomplished by introducing ethanolamine (ETAM) as the co-solvent blending deionized water while citric acid and glycine are used as the main carbon source and bridge for condensation respectively. The size distribution of ETAM-GQDs was determined by AFM and TEM. The photoluminescent (PL) quantum yield of ETAM-GQDs was measured to be 38.2% under 365 nm light excitation by using quinine sulphate as a standard. The biocompatibility of ETAM-GQDs evaluated by MTT assay indicated the low toxicity of ETAM-GQDs at concentrations below 1 mg/mL. Fluorescent imaging of living HL7702 cells incubated with ETAM-GQDs demonstrated that ETAM-GQDs can be applied as an effective bioimaging agent.