文章信息/Info

Title:

Research Progress in Nanopores-Based Sensing Technology for Nucleic Acid Detection

作者:

汪榮亮; 谷德健; 劉全俊

東南大學(xué)生物科學(xué)與醫(yī)學(xué)工程學(xué)院

Author(s):

WANG Rongliang;  GU Dejian;  LIU Quanjun

School of Biological Science and Medical Engineering, Southeast University

關(guān)鍵詞:

納米孔; 單分子檢測; 核酸檢測; DNA 測序; 重金屬離子檢測

Keywords:

nanopore;  single molecule detection;  nucleic acid detection;  DNA sequencing;  hevay metal ion detection

DOI:

10.7502/j.issn.1674-3962.2018.01.09

文獻(xiàn)標(biāo)志碼:

A

摘要:

核酸是儲存、復(fù)制和傳遞遺傳信息的主要物質(zhì)基礎(chǔ)，實(shí)現(xiàn)核酸的精準(zhǔn)、靈敏檢測對DNA損傷研究、基因治療、突變分析、細(xì)菌感染、藥物研發(fā)、臨床診斷等方面都具有重要意義。核酸傳感檢測有著廣泛的應(yīng)用前景，如環(huán)境、生物醫(yī)學(xué)、藥學(xué)、食品、法醫(yī)學(xué)等領(lǐng)域。傳統(tǒng)的檢測方法有光學(xué)、質(zhì)量型壓電DNA傳感、電化學(xué)傳感、表面等離子共振傳感檢測等。隨著基因工程技術(shù)的飛速發(fā)展，許多交叉學(xué)科新的分析手段在生命科學(xué)領(lǐng)域的應(yīng)用引起廣泛關(guān)注。作為高靈敏的單分子檢測技術(shù)，納米孔傳感技術(shù)進(jìn)展飛快。因其獨(dú)特的物理和電學(xué)性質(zhì)，對生物分子的檢測有快速、無需標(biāo)記和擴(kuò)增等優(yōu)點(diǎn)，已被廣泛研究證明。從DNA測序、DNA甲基化檢測、DNA損傷檢測、DNA-蛋白相互作用、重金屬離子檢測、RNA、病毒檢測等方面分別介紹納米孔傳感技術(shù)在核酸檢測方向的研究進(jìn)展。

Abstract:

Nucleic acid is the major physical basis of genetic information for storage, replication and transmission. Realizing accurate and sensitive detection of nucleic acid plays an important role for DNA lesion analysis, gene therapy, mutation analysis, bacterial infection, R&D of drugs, clinical diagnosis and so on. DNA sensing has a wide range of applications, such as environment, biomedicine, pharmaceutics, food, forensic medicine and other fields. Traditional methods mainly include optics, piezoelectric deoxyribonucleic acid sensing, electrochemical deoxyribonucleic acid sensing and surface plasmon resonance. With the rapid developments of genetic engineering technology, many new interdiscipline analytical tools in the field of life science have attracted widespread attentions. As a high-sensitive single molecule detection technology, nanopore sensing technology has got a fast development pace. Nanopores, with its unique physical and electrical properties, detect bio-molecules rapidly without labeling or amplification, and have been proven to be very useful. The progress of nanopore�瞓ased sensing technology for nucleic acid detection about DNA sequencing, DNA methylation detection, DNA lesion detection, Protein-DNA interaction, hevay metal ion detection, RNA and virus detection are reviewed.