[1]聞鄭峰,朱君,管軍,等.氟碳乳劑的制備及其對機體供氧能力研究[J].中國材料進展,2016,(6):041-45.[doi:10.7502/j.issn.1674-3962.2016.06.09]
WEN Zhengfeng,ZHU Jun,GUAN Jun,et al.The Preparation of Perfluorocarbon Emulsion and Its Ability of Oxygen Supply to Organism[J].MATERIALS CHINA,2016,(6):041-45.[doi:10.7502/j.issn.1674-3962.2016.06.09]
點擊復制
氟碳乳劑的制備及其對機體供氧能力研究(
)
中國材料進展[ISSN:1674-3962/CN:61-1473/TG]
- 卷:
-
- 期數:
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2016年第6期
- 頁碼:
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041-45
- 欄目:
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特約研究論文
- 出版日期:
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2016-06-30
文章信息/Info
- Title:
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The Preparation of Perfluorocarbon Emulsion and Its Ability of Oxygen Supply to Organism
- 作者:
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聞鄭峰1; 朱君2; 管軍1; 姚燕杰2; 劉恬2; 何丹農2; 林兆奮1
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（1. 第二軍醫大學附屬長征醫院急救科，
（2. 納米技術及應用國家工程研究中心，
- Author(s):
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WEN Zhengfeng1; ZHU Jun2; GUAN Jun1; YAO Yanjie2; LIU Tian2; HE Dannong2; LIN Zhaofen1
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（1. Department of Emergency, Changzheng Hospital, The Second Military Medical University
（2. National Engineering Research Center for Nanotechnology
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- 關鍵詞:
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全氟化碳; 人工肺表面活性物質; 細胞增殖; 肺部氧合
- DOI:
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10.7502/j.issn.1674-3962.2016.06.09
- 文獻標志碼:
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A
- 摘要:
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探究了以人工肺表面活性物質（APS）為載體的氟碳乳劑對肺癌細胞（A549）生長的影響以及通過肺部給藥后對海水淹溺動物肺部氧合能力的改善情況。制備得到了分散性較好的氟碳乳劑，其平均粒徑為46.62 nm。通過核磁共振及離子色譜證明了氟碳成功地包封其中，氟碳的含量為23.54%。通過A549細胞共孵育實驗，證明了氟碳乳劑能在1%氧濃度下較明顯地促進A549細胞增殖；當氟碳乳劑濃度為5 mg/mL時，細胞增殖率達124%，而在正常氧濃度下，其對A549細胞增殖無明顯作用。實驗兔的海水淹溺模型實驗證明了，海水淹溺實驗兔氟碳乳劑肺部給藥后血氧分壓迅速恢復，氟碳乳劑具有一定改善動物肺部氧合的能力。氟碳乳劑在低氧下能促進A549細胞增殖，通過肺部給藥后對海水淹溺動物肺部氧合能力有一定的改善，這說明其在通過肺部給藥來改善肺部通氣應用中有潛在的應用價值。
- Abstract:
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Artificial pulmonary surfactant (APS)/Perfluorocarbon (PFC) emulsion using APS as a carrier is prepared through highspeed emulsification, the effect of APS/PFC emulsion on cell proliferation and its ability of improving pulmonary oxygenation to seawater drowning rabbits are explored. PFC content of APS/PFOB freezedried powder is 23.54%. A549 cell viability is increased gradually with the increasing concentration of APS/PFOB emulsion under condition of 1% oxygen concentration. About 124% cell proliferation rate is maintained when the concentration is 5 mg/mL. Moreover, it is demonstrated that APS/PFOB emulsion can improve pulmonary oxygenation to seawater drowning rabbits through pulmonary delivery. APS/PFC emulsion has abilities of promoting A549 cell proliferation during hypoxia and improving pulmonary oxygenation to seawater drowning rabbits through pulmonary delivery, it has potential application for improving lung ventilation.
更新日期/Last Update:
2016-07-12