文章信息/Info

Title:

Research Progress of Hybridization Chain Reaction Combined with Nanomaterials for Nucleic Acid Detection

文章編號:

1674-3962(2020)04-0325-07

作者:

劉永新; 袁旦; 陳沁; 鈕冰

（上海大學生命科學學院，上海市 200444）

Author(s):

LIU Yongxin;  YUAN Dan;  CHEN Qin;  NIU Bing

(School of Life Science, Shanghai University, Shanghai 200444, China)

關鍵詞:

雜交鏈式反應（HCR）; 銀納米顆粒; 金納米顆粒; 氧化石墨烯; 納米材料; 核酸檢測

Keywords:

hybridization chain reaction (HCR);  silver nanoparticles;  gold nanoparticles;  graphene oxide;  nanomaterials;  nucleic acid detection

分類號:

R318.08

DOI:

10.7502/j.issn.1674-3962.201901016

文獻標志碼:

A

摘要:

核酸檢測在疾病早期診斷、分期、治療監測、預后判斷以及產前基因診斷等許多方面有著重要意義，然而目前對核酸的檢測技術不僅存在耗時長、成本高等問題，而且工作量大、操作繁瑣，嚴重影響其在診斷中的應用。雜交鏈式反應（hybridization chain reaction, HCR）是一種新型信號擴增技術，它通過單鏈DNA（ssDNA）引發兩種穩定的DNA發夾探針在恒溫下發生雜交反應，從而放大檢測信號，實現選擇性檢測靶分子的目的。不僅如此，HCR還可以結合不同的納米材料用于檢測各種目標物如核酸、蛋白質、細胞、金屬離子等等。對HCR技術結合幾種常見的納米材料（銀納米顆粒、金納米顆粒、氧化石墨烯以及幾種其他納米材料）用于核酸的檢測進行了總結，并對今后的發展趨勢進行了展望。

Abstract:

Nucleic acid detection is of great significance in many aspects such as early diagnosis, staging, treatment monitoring, prognosis judgment and prenatal gene diagnosis. However, the current nucleic acid testing technology is not only time-consuming and costly, but also has a large workload and cumbersome operation, which significantly affects its application in diagnosis. Hybridization chain reaction (HCR) is a new type of signal amplification technology, which induces two stable DNA hairpin probes to hybridize under constant temperature through single chain DNA (ssDNA), thus amplifying the detection signal and achieving the goal of selective detection of target molecules. Moreover, the hybrid chain reaction can also bind different nanomaterials to detect various target substances such as nucleic acids, proteins, cells, metal ions and so on. In this paper, HCR technology combined with several common nanomaterials (silver nanoparticles, gold nanoparticles, graphene oxide and several other nanomaterials) for nucleic acid detection was summarized, and the future development trend was prospected.