文章信息/Info

Title:

The Compatibility in vitro and in vivo of Novel Biomedical Magnesium Alloy

文章編號:

1674-3962(2022)11-0959-06

作者:

高質涵1; 高銘澤2; 孫藝3; 劉海峰1; 付高潔4; 肖陽4

（1. 牡丹江醫學院醫藥研究中心，黑龍江 牡丹江 157011）（2. 牡丹江醫學院醫學影像學院，黑龍江 牡丹江 157011）（3. 東北財經大學工商管理學院，遼寧 大連 116000）（4. 牡丹江醫學院附屬第二醫院，黑龍江 牡丹江 157011）

Author(s):

GAO Zhihan1; GAO Mingze2; SUN Yi3; LIU Haifeng1; FU Gaojie4; XIAO Yang4

（1. Medical Research Center, Mudanjiang Medical University, Mudanjiang 157011,China）（2. School of Medical Imaging, Mudanjiang Medical University, Mudanjiang 157011, China）（3. School of Business Administration, Dongbei University of Finance and Economics, Dalian 116000, China）（4. The Second Affiliated Hospital, Mudanjiang Medical University, Mudanjiang 157011, China)

關鍵詞:

鎂合金; 鎵; 鍶; 生物相容; 生物降解; 骨科植入物

Keywords:

magnesium alloy;  gallium;  strontium;  biocompatibility;  biodegradation;  orthopedic implants

分類號:

R318.08

DOI:

10.7502/j.issn.1674-3962.202101017

文獻標志碼:

A

摘要:

近年來，鎂合金材料由于其生物可降解、且機械性能與皮質骨相似而備受關注。探討了含不同質量分數鎵和鍶元素的鎂合金材料在體內外的相容性及是否具有促進骨愈合的能力。將各組材料在杜氏改良Eagle培養基（DMEM）中與人骨髓間充質干細胞（hBMSCs）共同培養，然后通過熒光染色判斷材料在體外的細胞毒性；將材料植入大鼠股骨內并通過X射線透視、Micro�睠T評價植入材料在體內的相容性以及作用，通過掃描電鏡觀察植入材料表面形貌的改變。研究結果表明，相較于純鈦，鎂合金材料會對細胞增殖產生一定的抑制作用；含鍶的鎂合金材料具有更好的促成骨能力，鎂合金材料的降解速率小于純鎂。

Abstract:

In recent years, magnesium-based alloys have attracted extensive attention due to their biodegradability and mechanical properties similarity with those of cortical bone. This paper investigated the compatibility in vitro and in vivo of magnesium alloys containing different amounts of gallium and strontium and whether they have the ability of promoting bone healing. The materials of each group were co-cultured with human bone marrow mesenchymal stem cells (hBMSCs) in Duchenne modified eagle medium (DMEM), and the cytotoxicity of the materials in vitro was evaluated by fluorescence staining. The materials were implanted into the femur of rats, and X’ray fluoroscopy and Micro-CT were used to evaluate the compatibility in vivo and effect of the planted materials, SEM was used to observe the changes of the surface morphology of the planted materials. Compared with pure titanium, magnesium alloys have a certain inhibitory effect on cells proliferation. The alloy material containing strontium has better bone forming ability, and the degradation rate is lower than that of pure magnesium.