文章信息/Info

Title:

Advance of Protease Targeted Fluorescent Theranostic Nanoprobes

文章編號:

1674-3962(2022)09-0689-08

作者:

邢潔1; 2; 姚俊列1; 2; 3; 馬雪華1; 2; 3; 吳愛國1; 2

（1.中國科學院寧波材料技術與工程研究所 寧波慈溪生物醫學工程研究所 浙江省生物醫學材料技術與應用國際科技合作基地 中國科學院磁性材料與器件重點實驗室 醫用植介入材料浙江省工程研究中心，浙江 寧波 315201）（2.先進能源科學與技術廣東省實驗室，廣東 惠州516000）（3.中國科學院大學，北京 100049）

Author(s):

XING Jie1; 2; YAO Junlie1; 2; 3; MA Xuehua1; 2; 3; WU Aiguo1; 2

(1. Zhejiang Biomedical Materials Technology and Application International Science and Technology Cooperation Base, Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for ImplantInterventional Materials, Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technologyand Engineering, Chinese Academy of Sciences, Ningbo 315201, China)

關鍵詞:

納米技術; 熒光探針; 基質金屬蛋白酶; 組織蛋白酶; 熒光成像

Keywords:

nanotechnology;  fluorescent probe;  matrix metalloproteinases;  cathepsin;  fluorescence imaging

分類號:

TB383.1;R318.08; R730.4

DOI:

10.7502/j.issn.1674-3962.202112030

文獻標志碼:

A

摘要:

蛋白酶是生物系統生長發育、營養代謝等活動中必不可少的基礎大分子催化物質，是生命醫學領域一直以來關注的重點。研究表明，大多數疾病的發生伴隨著蛋白酶的表達異常，某些特定蛋白酶成為一些疾病的生理標志物，因此開發蛋白酶響應診療探針可對相應的疾病診斷和治療提供幫助。熒光成像具有實時、無創、空間分辨率高等特性，而納米診療探針通過合理設計可實現蛋白酶響應熒光成像的同時整合其它治療方式于同一納米平臺，達到成像和治療一體化的良好效果。總結了靶向基質金屬蛋白酶、組織蛋白酶、半胱天冬氨酸蛋白酶、胰蛋白酶和一些其它類型（如成纖維細胞激活蛋白等）蛋白酶的納米熒光診療探針的成像設計原理及生物醫學應用，希望促進新型低毒、高性能的蛋白酶響應型納米熒光探針的開發，并早日實現臨床轉化。

Abstract:

Protease is an essential basic macromolecular catalyst for the growth and development of biological system and nutritional and metabolic activities. It has always been the focus of life medicine and other fields. Studies have shown that most diseases are accompanied by abnormal expression of proteases, so some specific proteases have become physiological markers of some diseases. The development of protease detection tools can help in the diagnosis and treatment of corresponding diseases. Fluorescence imaging has the characteristics of real-time, noninvasive and high spatial resolution. Through reasonable design, the fluorescent nano diagnosis and treatment probe can realize the response imaging of protease precise targeting. At the same time, based on nano structure, it can combine other treatment methods on the platform to achieve the good effect of imaging and treatment integration. In this review, we summarize the imaging design principles and biomedical applications of nano fluorescent diagnostic probes targeted to matrix metalloproteinases, cathepsins, caspases, trypsin and some other types of proteases such as fibroblast activating protein, hoping to promote the production of new protease responsive nano fluorescent probes with low toxicity and high performance, and realize clinical transformation as soon as possible.