文章信息/Info

Title:

A Review on Functional Micro/Nano-Materials for Mass Cytometry

文章編號:

1674-3962(2025)07-0648-08

作者:

雷婷玥; 李萬萬

上海交通大學材料科學與工程學院，上海 200240

Author(s):

LEI Tingyue;  LI Wanwan

School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

關鍵詞:

質譜流式技術; 質譜流式細胞儀; 質量標簽試劑; 功能微納米材料

Keywords:

mass cytometry;  cytometer time of flight;  mass tag reagent;  functional micro/nano-materials

分類號:

TB383;0657

DOI:

10.7502/j.issn.1674-3962.202310015

文獻標志碼:

A

摘要:

質譜流式技術（mass cytometry，MC）是一種新興的生物分析技術，該技術通過質譜流式細胞儀（CyTOF）中電感耦合等離子體飛行時間質譜（ICP-TOF-MS）分析儀進行檢測，其中作為質量標簽的功能微納米材料可同時標記、測量多達50種生物標志物，因此該技術能夠在單細胞水平識別、量化細胞系統的各種參數。隨著新材料技術的發展，通過CyTOF獲得的信息范圍不斷增加，而其中典型的功能微納米材料主要包括金屬螯合聚合物（metal chelating polymers, MCP）、金屬納米顆粒(metal nanoparticles, MNP)和聚合物微球等。介紹、總結了這幾種功能微納米材料作為質量標簽試劑的主要性能、特點和研究現狀，并對其未來的發展方向做了簡單評述，以助力質譜流式技術的發展。

Abstract:

Mass cytometry (MC) is an emerging biological analysis technique which detected by inductively coupled plasma time of flight mass spectrometry (ICP-TOF-MS). Functional micro/nano-material as mass tag makes simultaneous labeling and measurement for up to 50 biomarkers a reality. Therefore, this technology can recognize and quantify different biomarkers at single cell level.With the development of new materials serving as mass tag, the range of information obtained through CyTOF continues to increase. Typical functional micro/nano-materials include metal chelating polymers (MCP), metal nanoparticles（MNP） and polymer microspheres. This article introduces and summarizes the main characteristics and applications of these materials as mass tag reagents, and provides a brief review of their future development to assist the progress of mass cytometry.